pe anti mouse ly6g ly6c gr 1 antibody  (Elabscience Biotechnology)

Journal: STAR Protocols

Article Title: Protocol to study the immune profile of syngeneic mouse tumor models

doi: 10.1016/j.xpro.2024.103139

Figure Lengend Snippet: Antibody panel to identify myeloid cell populations

Article Snippet: Rat monoclonal anti-mouse Gr-1 (Ly6G/Ly6C) (clone RB6-8C5) PE-Cyanine7 (1:200) , BD Biosciences , Cat #: 565033; RRID: AB_2739049.

Techniques: Staining

Journal: STAR Protocols

Article Title: Protocol to study the immune profile of syngeneic mouse tumor models

doi: 10.1016/j.xpro.2024.103139

Figure Lengend Snippet:

Article Snippet: Rat monoclonal anti-mouse Gr-1 (Ly6G/Ly6C) (clone RB6-8C5) PE-Cyanine7 (1:200) , BD Biosciences , Cat #: 565033; RRID: AB_2739049.

Techniques: Recombinant, Staining, Software, Sterility, Flow Cytometry

Journal: Cell reports

Article Title: Reprogramming of tumor-associated macrophages via NEDD4-mediated CSF1R degradation by targeting USP18

doi: 10.1016/j.celrep.2023.113560

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rat monoclonal anti-mouse Gr-1 (Ly6G/Ly6C) (clone RB6-8C5) PE-Cyanine7 , BD Biosciences , Cat #: 565033; RRID: AB_2739049.

Techniques: Recombinant, Modification, Staining, Lysis, Protease Inhibitor, Suspension, Knock-Out, Plasmid Preparation, Expressing, Mutagenesis, shRNA, Negative Control, Software, Flow Cytometry, Imaging

Journal: Cell Reports Medicine

Article Title: Venetoclax, alone and in combination with the BH3 mimetic S63845, depletes HIV-1 latently infected cells and delays rebound in humanized mice

doi: 10.1016/j.xcrm.2023.101178

Figure Lengend Snippet:

Article Snippet: PE Rat anti-Mouse LY6C + LY6G (Gr-1) , BD Pharmingen , Cat# 553128; RRID: AB_394644.

Techniques: Virus, Recombinant, In Vivo, Ex Vivo, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Staining, Cell Isolation, Expressing, Software

Journal: Infection and Immunity

Article Title: Elevated Levels of Interleukin-27 in Early Life Compromise Protective Immunity in a Mouse Model of Gram-Negative Neonatal Sepsis

doi: 10.1128/IAI.00828-19

Figure Lengend Snippet: Cellular profiling of IL-27 producers in the spleen. Neonatal C57BL/6 (WT) mice were subcutaneously inoculated with a target inoculum of ∼2 × 10 6 CFU/mouse of E. coli O1:K1:H7 or PBS as a control on day 3 or 4 of life. At 10 or 24 h postinfection, mice were sacrificed, and spleens were harvested. Single-cell suspensions of splenocytes were immunolabeled for cell surface markers Gr-1, F4/80, CD11c, or CD115 and intracellular IL-27p28. Cells were analyzed by flow cytometry. Figures represent combined results from 2 to 3 independent experiments. (A) Representative dot plots of splenocytes labeled at 10 h postinfection for the indicated marker are shown. Phycoerythrin (PE)-stained cell surface markers are represented on the y axis and fluorescein isothiocyanate (FITC)-stained IL-27p28 signal is represented on the x axis for each dot plot. (B and D) The percentage that is double positive (upper right quadrant) of the population for each cell surface marker in control (CT) and E. coli -infected (Ifx) spleens at 10 h (B) or 24 h (D) postinfection. (C and E) Percent change in mean fluorescence intensity (MFI) of fluorescein isothiocyanate (FITC) signal that corresponds to the IL-27p28 protein in the double-positive population for infected relative to control cells at 10 h (C) or 24 h (E) postinfection. (B, D) Statistical assessment was performed using a one-way analysis of variance (ANOVA) with Dunnett’s multiple comparison test. Means ± standard error are displayed. (C, E) Mean changes ± standard error in absolute values of MFI cell surface marker percentages at 10 h (C) and 24 h (E) postinfection in splenocytes were analyzed relative to a normalized baseline within the control groups using individual, unpaired t tests for each cell surface marker. (C) Asterisks indicate the following significant differences between infected and control splenocytes at 10 h postinfection: Gr1 ( P = 0.0003), F4/80 ( P = 0.0027), CD11c ( P = 0.0237), and CD115 ( P < 0.0001). (E) At 24 h postinfection, the asterisk indicates significance for CD11c ( P = 0.0111). Results shown for Gr-1 ( P = 0.08) and F4/80 ( P = 0.0689) were trending toward significance.

Article Snippet: Cell surface markers were immunolabeled with anti-Gr-1 (PE rat anti-mouse Ly6G and Ly6C; BD Pharmingen, Franklin, NJ), F4/80 (anti-F4/80 PE; Miltenyi Biotec), CD11c (CD11c-PE; Miltenyi Biotec), or CD115 (CD115-PE; Miltenyi Biotec), washed, and fixed with 3% paraformaldehyde.

Techniques: Immunolabeling, Flow Cytometry, Labeling, Marker, Staining, Infection, Fluorescence

Journal: Infection and Immunity

Article Title: Elevated Levels of Interleukin-27 in Early Life Compromise Protective Immunity in a Mouse Model of Gram-Negative Neonatal Sepsis

doi: 10.1128/IAI.00828-19

Figure Lengend Snippet: Cellular profiling of IL-27 producers in the blood. Neonatal C57BL/6 (WT) mice were subcutaneously inoculated with a target inoculum of ∼2 × 10 6 CFU/mouse of E. coli O1:K1:H7 or PBS as a control on day 3 or 4 of life. At 10 or 24 h postinfection, mice were sacrificed, and blood was harvested and pooled for control and infected pups. PBMCs obtained by Ficoll density gradient centrifugation were immunolabeled for cell surface markers Gr-1, F4/80, CD11c, or CD115 and intracellular IL-27p28. Cells were analyzed by flow cytometry. Figures represent combined results from 2 to 3 independent experiments. (A) Representative dot plots of infected splenocytes at 10 h postinfection are labeled for the indicated marker are shown. Phycoerythrin (PE)-stained cell surface markers are represented on the y axis and fluorescein isothiocyanate (FITC)-stained IL-27p28 signal is represented on the x axis for each dot plot. (B and D) The percentage that is double positive (upper right quadrant) of the population for each cell surface marker in control (CT) and E. coli -infected (Ifx) spleens at 10 h (B) or 24 h (D) postinfection. (C and E) Percent change in mean fluorescence intensity (MFI) of fluorescein isothiocyanate (FITC) signal that corresponds to the IL-27p28 protein in the double-positive population for infected relative to control cells at 10 h (C) or 24 h (E) postinfection. (B, D) Statistical assessment was performed using a one-way analysis of variance (ANOVA) with Dunnett’s multiple-comparison test. Mean changes ± standard error are displayed. (C, E) Mean changes ± standard error in absolute values of MFI cell surface marker percentages at 10 h (C) and 24 h (E) postinfection in splenocytes were analyzed relative to a normalized baseline within the control groups using individual, unpaired t tests for each cell surface marker. (C) Asterisks indicate the following significant differences between infected and control splenocytes at 10 h postinfection: Gr1 ( P = 0.0119) and F4/80 ( P = 0.0056). Results shown for CD11c ( P = 0.0850) at 10 h postinfection were trending toward significance. (E) At 24 h postinfection, the asterisk indicates significance for CD11c ( P = 0.0345).

Article Snippet: Cell surface markers were immunolabeled with anti-Gr-1 (PE rat anti-mouse Ly6G and Ly6C; BD Pharmingen, Franklin, NJ), F4/80 (anti-F4/80 PE; Miltenyi Biotec), CD11c (CD11c-PE; Miltenyi Biotec), or CD115 (CD115-PE; Miltenyi Biotec), washed, and fixed with 3% paraformaldehyde.

Techniques: Infection, Gradient Centrifugation, Immunolabeling, Flow Cytometry, Labeling, Marker, Staining, Fluorescence